Composite
Part:BBa_M45125:Design
Designed by: Sherissa Ward Group: UtahState_BE5930_-_S14 (2014-04-21)
F-Plasmid OriT with Autolysin for Cell Death Induced Conjugation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 234
Illegal NheI site found at 280 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 381
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There may need to be up-regulation of the autolysin in this case to increase the rate of cell death.
Source
This part was made using an autolysin from Bacillus subtilis and other parts in the registry.