Composite

Part:BBa_M45125:Design

Designed by: Sherissa Ward   Group: UtahState_BE5930_-_S14   (2014-04-21)

F-Plasmid OriT with Autolysin for Cell Death Induced Conjugation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 234
    Illegal NheI site found at 280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There may need to be up-regulation of the autolysin in this case to increase the rate of cell death.


Source

This part was made using an autolysin from Bacillus subtilis and other parts in the registry.

References